sterile deionized water (Bio-Rad)
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sterile deionized water
Sterile Deionized Water, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 504 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile+deionized+water/Sterile+Water/pmc12484763-81-56-61
Average 94 stars, based on 504 article reviews
Sterile Deionized Water, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 504 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile+deionized+water/Sterile+Water/pmc12484763-81-56-61
Average 94 stars, based on 504 article reviews
sterile deionized water - by Bioz Stars,
2026-09
94/100 stars
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Polymerase Chain Reaction:Article Title: Soil Organic Carbon Attenuates the Influence of Plants on Root-Associated Bacterial Community Article Snippet: The qPCR assays were conducted in triplicate on a CFX96 Optical Real-Time Detection System (Bio-Rad Laboratories, Inc. United States). .. The PCR reaction solution contained 10 μl 2 × PCR buffer (iQ TM SYBR Green Supermix, Bio-Rad), 1 μl of each primer (primer set Eub338/Eub518, 10 μM), 1 μl of template DNA, and Article Title: Serotonin depletion during the postnatal developmental period causes behavioral and cognitive alterations and decreases BDNF level in the brain of rats. Article Snippet: © 2021 International Society for Developmental Neuroscience 1Department of Physiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran 2Pharmaceutical Sciences Research Center, Ardabil University of Medical Sciences, Ardabil, Iran 3Students Research Committee, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran 4Department of Anatomical Sciences and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran Article Title: Sequencing, assembly, annotation, and gene expression: novel insights into browning-resistant Luffa cylindrica Article Snippet: .. RT-qPCR was performed on Bio-Rad CFX96 PCR platform (Bio-Rad, USA) using Hieff qPCR SYBR Green Master Mix (Yeason, Shanghai, China).The reaction mixture contains 10 μL of enzyme, 2 μL of diluted cDNA, 0.4 μL of forward or reverse primer, and SYBR Green Assay:Article Title: Soil Organic Carbon Attenuates the Influence of Plants on Root-Associated Bacterial Community Article Snippet: The qPCR assays were conducted in triplicate on a CFX96 Optical Real-Time Detection System (Bio-Rad Laboratories, Inc. United States). .. The PCR reaction solution contained 10 μl 2 × PCR buffer (iQ TM SYBR Green Supermix, Bio-Rad), 1 μl of each primer (primer set Eub338/Eub518, 10 μM), 1 μl of template DNA, and Article Title: Sequencing, assembly, annotation, and gene expression: novel insights into browning-resistant Luffa cylindrica Article Snippet: .. RT-qPCR was performed on Bio-Rad CFX96 PCR platform (Bio-Rad, USA) using Hieff qPCR SYBR Green Master Mix (Yeason, Shanghai, China).The reaction mixture contains 10 μL of enzyme, 2 μL of diluted cDNA, 0.4 μL of forward or reverse primer, and Article Title: The Ground-Based BIOMEX Experiment Verification Tests for Life Detection on Mars Article Snippet: The qPCR reactions were performed in the Bio Rad CFX96 Real-Time PCR Detection System, and the Bio Rad CFX Manager software processed the results in graphs. .. The reactions were carried out in triplicate in the 96-well plate with a solution containing 7.5 μL iQ SYBR Green Supermix (Bio Rad, Milan, Italy), 1.0 μL each primer solution (5 pmol/μL), and 5.5 ng DNA template in a final volume of 15 μL; for the No Template Control (NTC) sample, 5.5 μL Sterility:Article Title: Soil Organic Carbon Attenuates the Influence of Plants on Root-Associated Bacterial Community Article Snippet: The qPCR assays were conducted in triplicate on a CFX96 Optical Real-Time Detection System (Bio-Rad Laboratories, Inc. United States). .. The PCR reaction solution contained 10 μl 2 × PCR buffer (iQ TM SYBR Green Supermix, Bio-Rad), 1 μl of each primer (primer set Eub338/Eub518, 10 μM), 1 μl of template DNA, and Article Title: Using environmental DNA methods to improve detectability in an endangered sturgeon ( Acipenser sinensis ) monitoring program Article Snippet: The Chinese sturgeon eDNA was detected and quantified by a Bio-Rad QX200TM AutoDGTM Droplet DigitalTM PCR System at the Analysis and Testing Center in Institute of Hydrobiology, Chinese Academy of Sciences. .. We performed the ddPCR amplification in 20 μl reaction volume containing 2 μl water sample genomic DNA, 2 μl of each primer (1 μM), 4 μl Article Title: 18β-Glycyrrhetinic acid altered the intestinal permeability in the human Caco-2 monolayer cell model. Article Snippet: Purpose Glycyrrhizin (GL) and its metabolites 18α-glycyrrhetinic acid (18α-GA) and 18β-glycyrrhetinic acid (18β-GA) are used as traditional medicine and food sweeteners.. As the major rout of their administration is oral way, therefore their impact on intestinal epithelial cells are investigated.. Methods The effects of GL and its metabolites on cell viability using MTT assay, on cytotoxicity using LDH release, on integrity of intestinal epithelial cells by measuring the transepithelial electrical resistance (TEER) and Luciferase permeability tests, on the expression of tight junction proteins at mRNA and protein level by qPCR and western blot techniques, and ultimately on the rate of test compounds absorption via Caco-2 cells monolayer were investigated. Article Title: Concurrent Optimizations of Efficacy and Blood-Brain Barrier Permeability in New Macrocyclic LRRK2 Inhibitors for Potential Parkinson's Disease Therapeutics. Article Snippet: The elevated activity of leucine-rich repeat kinase 2 (LRRK2) is implicated in the pathogenesis of Parkinson’s disease (PD).. The quest for effective LRRK2 inhibitors has been impeded by the formidable challenge of crossing the blood−brain barrier (BBB).. We leveraged structure-based de novo design and developed robust three-dimensional quantitative structure−activity relationship (3D-QSAR) models to predict BBB permeability, enhancing the likelihood of the inhibitor’s brain accessibility. Article Title: Serotonin depletion during the postnatal developmental period causes behavioral and cognitive alterations and decreases BDNF level in the brain of rats. Article Snippet: © 2021 International Society for Developmental Neuroscience 1Department of Physiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran 2Pharmaceutical Sciences Research Center, Ardabil University of Medical Sciences, Ardabil, Iran 3Students Research Committee, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran 4Department of Anatomical Sciences and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran Article Title: Sequencing, assembly, annotation, and gene expression: novel insights into browning-resistant Luffa cylindrica Article Snippet: .. RT-qPCR was performed on Bio-Rad CFX96 PCR platform (Bio-Rad, USA) using Hieff qPCR SYBR Green Master Mix (Yeason, Shanghai, China).The reaction mixture contains 10 μL of enzyme, 2 μL of diluted cDNA, 0.4 μL of forward or reverse primer, and Article Title: The Ground-Based BIOMEX Experiment Verification Tests for Life Detection on Mars Article Snippet: The qPCR reactions were performed in the Bio Rad CFX96 Real-Time PCR Detection System, and the Bio Rad CFX Manager software processed the results in graphs. .. The reactions were carried out in triplicate in the 96-well plate with a solution containing 7.5 μL iQ SYBR Green Supermix (Bio Rad, Milan, Italy), 1.0 μL each primer solution (5 pmol/μL), and 5.5 ng DNA template in a final volume of 15 μL; for the No Template Control (NTC) sample, 5.5 μL Amplification:Article Title: Uncovering Cercospora species affecting sugar beet in Iran with rapid and accurate detection of C. beticola using LAMP assay Article Snippet: .. PCR amplification of the actA , gapdh , his3 , cmdA , and tef1 genomic regions was performed in 25 μL reaction volumes containing 5–10 ng of genomic DNA, 1 × reaction buffer, 2 mM MgCl2, 40 μM of each dNTP, 0.7 μL DMSO, 0.2 μM of each primer, 0.4 units of Taq DNA polymerase, and Article Title: Using environmental DNA methods to improve detectability in an endangered sturgeon ( Acipenser sinensis ) monitoring program Article Snippet: The Chinese sturgeon eDNA was detected and quantified by a Bio-Rad QX200TM AutoDGTM Droplet DigitalTM PCR System at the Analysis and Testing Center in Institute of Hydrobiology, Chinese Academy of Sciences. .. We performed the ddPCR amplification in 20 μl reaction volume containing 2 μl water sample genomic DNA, 2 μl of each primer (1 μM), 4 μl Concentration Assay:Article Title: 18β-Glycyrrhetinic acid altered the intestinal permeability in the human Caco-2 monolayer cell model. Article Snippet: Purpose Glycyrrhizin (GL) and its metabolites 18α-glycyrrhetinic acid (18α-GA) and 18β-glycyrrhetinic acid (18β-GA) are used as traditional medicine and food sweeteners.. As the major rout of their administration is oral way, therefore their impact on intestinal epithelial cells are investigated.. Methods The effects of GL and its metabolites on cell viability using MTT assay, on cytotoxicity using LDH release, on integrity of intestinal epithelial cells by measuring the transepithelial electrical resistance (TEER) and Luciferase permeability tests, on the expression of tight junction proteins at mRNA and protein level by qPCR and western blot techniques, and ultimately on the rate of test compounds absorption via Caco-2 cells monolayer were investigated. Article Title: Concurrent Optimizations of Efficacy and Blood-Brain Barrier Permeability in New Macrocyclic LRRK2 Inhibitors for Potential Parkinson's Disease Therapeutics. Article Snippet: The elevated activity of leucine-rich repeat kinase 2 (LRRK2) is implicated in the pathogenesis of Parkinson’s disease (PD).. The quest for effective LRRK2 inhibitors has been impeded by the formidable challenge of crossing the blood−brain barrier (BBB).. We leveraged structure-based de novo design and developed robust three-dimensional quantitative structure−activity relationship (3D-QSAR) models to predict BBB permeability, enhancing the likelihood of the inhibitor’s brain accessibility. Quantitative RT-PCR:Article Title: 18β-Glycyrrhetinic acid altered the intestinal permeability in the human Caco-2 monolayer cell model. Article Snippet: Purpose Glycyrrhizin (GL) and its metabolites 18α-glycyrrhetinic acid (18α-GA) and 18β-glycyrrhetinic acid (18β-GA) are used as traditional medicine and food sweeteners.. As the major rout of their administration is oral way, therefore their impact on intestinal epithelial cells are investigated.. Methods The effects of GL and its metabolites on cell viability using MTT assay, on cytotoxicity using LDH release, on integrity of intestinal epithelial cells by measuring the transepithelial electrical resistance (TEER) and Luciferase permeability tests, on the expression of tight junction proteins at mRNA and protein level by qPCR and western blot techniques, and ultimately on the rate of test compounds absorption via Caco-2 cells monolayer were investigated. Article Title: Sequencing, assembly, annotation, and gene expression: novel insights into browning-resistant Luffa cylindrica Article Snippet: .. RT-qPCR was performed on Bio-Rad CFX96 PCR platform (Bio-Rad, USA) using Hieff qPCR SYBR Green Master Mix (Yeason, Shanghai, China).The reaction mixture contains 10 μL of enzyme, 2 μL of diluted cDNA, 0.4 μL of forward or reverse primer, and Real-time Polymerase Chain Reaction:Article Title: 18β-Glycyrrhetinic acid altered the intestinal permeability in the human Caco-2 monolayer cell model. Article Snippet: Purpose Glycyrrhizin (GL) and its metabolites 18α-glycyrrhetinic acid (18α-GA) and 18β-glycyrrhetinic acid (18β-GA) are used as traditional medicine and food sweeteners.. As the major rout of their administration is oral way, therefore their impact on intestinal epithelial cells are investigated.. Methods The effects of GL and its metabolites on cell viability using MTT assay, on cytotoxicity using LDH release, on integrity of intestinal epithelial cells by measuring the transepithelial electrical resistance (TEER) and Luciferase permeability tests, on the expression of tight junction proteins at mRNA and protein level by qPCR and western blot techniques, and ultimately on the rate of test compounds absorption via Caco-2 cells monolayer were investigated. Article Title: Sequencing, assembly, annotation, and gene expression: novel insights into browning-resistant Luffa cylindrica Article Snippet: .. RT-qPCR was performed on Bio-Rad CFX96 PCR platform (Bio-Rad, USA) using Hieff qPCR SYBR Green Master Mix (Yeason, Shanghai, China).The reaction mixture contains 10 μL of enzyme, 2 μL of diluted cDNA, 0.4 μL of forward or reverse primer, and Software:Article Title: 18β-Glycyrrhetinic acid altered the intestinal permeability in the human Caco-2 monolayer cell model. Article Snippet: Purpose Glycyrrhizin (GL) and its metabolites 18α-glycyrrhetinic acid (18α-GA) and 18β-glycyrrhetinic acid (18β-GA) are used as traditional medicine and food sweeteners.. As the major rout of their administration is oral way, therefore their impact on intestinal epithelial cells are investigated.. Methods The effects of GL and its metabolites on cell viability using MTT assay, on cytotoxicity using LDH release, on integrity of intestinal epithelial cells by measuring the transepithelial electrical resistance (TEER) and Luciferase permeability tests, on the expression of tight junction proteins at mRNA and protein level by qPCR and western blot techniques, and ultimately on the rate of test compounds absorption via Caco-2 cells monolayer were investigated. Western Blot:Article Title: Concurrent Optimizations of Efficacy and Blood-Brain Barrier Permeability in New Macrocyclic LRRK2 Inhibitors for Potential Parkinson's Disease Therapeutics. Article Snippet: The elevated activity of leucine-rich repeat kinase 2 (LRRK2) is implicated in the pathogenesis of Parkinson’s disease (PD).. The quest for effective LRRK2 inhibitors has been impeded by the formidable challenge of crossing the blood−brain barrier (BBB).. We leveraged structure-based de novo design and developed robust three-dimensional quantitative structure−activity relationship (3D-QSAR) models to predict BBB permeability, enhancing the likelihood of the inhibitor’s brain accessibility. SDS Page:Article Title: Concurrent Optimizations of Efficacy and Blood-Brain Barrier Permeability in New Macrocyclic LRRK2 Inhibitors for Potential Parkinson's Disease Therapeutics. Article Snippet: The elevated activity of leucine-rich repeat kinase 2 (LRRK2) is implicated in the pathogenesis of Parkinson’s disease (PD).. The quest for effective LRRK2 inhibitors has been impeded by the formidable challenge of crossing the blood−brain barrier (BBB).. We leveraged structure-based de novo design and developed robust three-dimensional quantitative structure−activity relationship (3D-QSAR) models to predict BBB permeability, enhancing the likelihood of the inhibitor’s brain accessibility. Control:Article Title: The Ground-Based BIOMEX Experiment Verification Tests for Life Detection on Mars Article Snippet: The qPCR reactions were performed in the Bio Rad CFX96 Real-Time PCR Detection System, and the Bio Rad CFX Manager software processed the results in graphs. .. The reactions were carried out in triplicate in the 96-well plate with a solution containing 7.5 μL iQ SYBR Green Supermix (Bio Rad, Milan, Italy), 1.0 μL each primer solution (5 pmol/μL), and 5.5 ng DNA template in a final volume of 15 μL; for the No Template Control (NTC) sample, 5.5 μL |